Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles

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Packaging HIV- or FIV-based lentivector expression constructs and transduction of VSV-G pseudotyped viral particles.

As with standard plasmid vectors, it is possible to transfect lentivectors in plasmid form into cells with low-to-medium efficiency to obtain transient expression of effectors. Packaging lentiviral expression constructs into pseudoviral particles, however, enables up to 100% transduction, even with difficult-to-transfect cells, such as primary, stem, and differentiated cells. Moreover, the lent...

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Cell- Cell Transmission of VSV-G Pseudotyped Lentivector Particles

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Minimum requirements for efficient transduction of dividing and nondividing cells by feline immunodeficiency virus vectors.

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ژورنال

عنوان ژورنال: Journal of Visualized Experiments

سال: 2012

ISSN: 1940-087X

DOI: 10.3791/3171